Detailed information of TA system TA237717

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39795..40064
• Replicon: plasmid pCY814036-KPC2
• Accession: NZ_CP093153
• Organism: Klebsiella pneumoniae strain CY814036

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MMZ10_RS28080
• Protein ID: WP_001372321.1
• Coordinates: 39939..40064 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39795..39860 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ10_RS28050	35505..36032	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
MMZ10_RS28055	36090..36323	+	234	WP_000006003.1	DUF905 family protein	-
MMZ10_RS28060	36384..38407	+	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
MMZ10_RS28065	38476..38910	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MMZ10_RS28070	38907..39626	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39638..39862	+	225	NuclAT_0	-	-
-	39795..39860	-	66	-	-	Antitoxin
MMZ10_RS28075	39848..39997	+	150	Protein_50	plasmid maintenance protein Mok	-
MMZ10_RS28080	39939..40064	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MMZ10_RS28085	40383..40679	-	297	Protein_52	hypothetical protein	-
MMZ10_RS28090	40979..41275	+	297	WP_001272251.1	hypothetical protein	-
MMZ10_RS28095	41386..42207	+	822	WP_001234445.1	DUF932 domain-containing protein	-
MMZ10_RS28100	42504..43151	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
MMZ10_RS28105	43428..43811	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MMZ10_RS28110	44002..44688	+	687	WP_015059009.1	PAS domain-containing protein	-
MMZ10_RS28115	44782..45009	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..140105	140105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T237717 WP_001372321.1 NZ_CP093153:39939-40064 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T237717 NZ_LR882500:c696741-696349 [Mycobacterium tuberculosis variant microti]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT237717 NZ_CP093153:c39860-39795 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin