Detailed information of TA system TA237691

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45785..46211
• Replicon: plasmid p104
• Accession: NZ_CP093070
• Organism: Escherichia coli strain BR1220

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MM431_RS25475
• Protein ID: WP_001372321.1
• Coordinates: 45785..45910 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45987..46211 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM431_RS25445 (41229)	41229..41918	-	690	WP_032173224.1	conjugal transfer transcriptional regulator TraJ	-
MM431_RS25450 (42105)	42105..42488	-	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MM431_RS25455 (42810)	42810..43412	+	603	WP_023908348.1	transglycosylase SLT domain-containing protein	-
MM431_RS25460 (43707)	43707..44528	-	822	WP_024214919.1	DUF932 domain-containing protein	-
MM431_RS25465 (44647)	44647..44934	-	288	WP_000107526.1	hypothetical protein	-
MM431_RS25470 (45235)	45235..45484	+	250	Protein_54	hypothetical protein	-
MM431_RS25475 (45785)	45785..45910	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MM431_RS25480 (45852)	45852..46001	-	150	Protein_56	plasmid maintenance protein Mok	-
- (45987)	45987..46211	-	225	NuclAT_0	-	Antitoxin
- (45987)	45987..46211	-	225	NuclAT_0	-	Antitoxin
- (45987)	45987..46211	-	225	NuclAT_0	-	Antitoxin
- (45987)	45987..46211	-	225	NuclAT_0	-	Antitoxin
MM431_RS25485 (46180)	46180..46942	-	763	Protein_57	plasmid SOS inhibition protein A	-
MM431_RS25490 (46939)	46939..47373	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
MM431_RS25495 (47428)	47428..49386	-	1959	Protein_59	ParB/RepB/Spo0J family partition protein	-
MM431_RS25500 (49445)	49445..49678	-	234	WP_000005970.1	DUF905 domain-containing protein	-
MM431_RS25505 (49734)	49734..50255	-	522	WP_000290849.1	single-stranded DNA-binding protein	-
MM431_RS25510 (50728)	50728..50970	-	243	WP_001365577.1	hypothetical protein	-
MM431_RS25515 (50898)	50898..51122	-	225	WP_072003755.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / aap/aspU / agg3B / agg3C / agg3D	1..104757	104757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T237691 WP_001372321.1 NZ_CP093070:c45910-45785 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T237691 NZ_LR882499:c2407403-2407086 [Mycobacterium tuberculosis variant microti OV254]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT237691 NZ_CP093070:c46211-45987 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin