Detailed information of TA system TA237687

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8369..8607
• Replicon: plasmid p104
• Accession: NZ_CP093070
• Organism: Escherichia coli strain BR1220

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: MM431_RS25265
• Protein ID: WP_023144756.1
• Coordinates: 8369..8503 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	8556..8607 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM431_RS25220 (3444)	3444..4613	+	1170	WP_032284272.1	hypothetical protein	-
MM431_RS25225 (4646)	4646..4774	+	129	Protein_6	IS481 family transposase	-
MM431_RS25230 (4899)	4899..5102	-	204	Protein_7	hypothetical protein	-
MM431_RS25235 (5208)	5208..5483	-	276	WP_032250674.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MM431_RS25240 (5483)	5483..5767	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	-
MM431_RS25245 (6681)	6681..7538	-	858	WP_032284166.1	incFII family plasmid replication initiator RepA	-
MM431_RS25250 (7531)	7531..7605	-	75	WP_031943482.1	RepA leader peptide Tap	-
MM431_RS25260 (7842)	7842..8096	-	255	WP_000083850.1	replication regulatory protein RepA	-
MM431_RS25265 (8369)	8369..8503	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (8556)	8556..8607	+	52	NuclAT_1	-	Antitoxin
- (8556)	8556..8607	+	52	NuclAT_1	-	Antitoxin
- (8556)	8556..8607	+	52	NuclAT_1	-	Antitoxin
- (8556)	8556..8607	+	52	NuclAT_1	-	Antitoxin
MM431_RS25270 (8574)	8574..8860	-	287	Protein_14	DUF2726 domain-containing protein	-
MM431_RS25275 (9372)	9372..9584	-	213	WP_001301409.1	ANR family transcriptional regulator	-
MM431_RS25280 (9716)	9716..10276	-	561	WP_000139341.1	fertility inhibition protein FinO	-
MM431_RS25285 (10331)	10331..11077	-	747	WP_000205736.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / aap/aspU / agg3B / agg3C / agg3D	1..104757	104757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T237687 WP_023144756.1 NZ_CP093070:c8503-8369 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T237687 NZ_LR882499:c2235236-2234892 [Mycobacterium tuberculosis variant microti OV254]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Nucleotide

• Length: 52 bp

>AT237687 NZ_CP093070:8556-8607 [Escherichia coli]
TCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin