Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3988420..3988961 Replicon chromosome
Accession NZ_CP093068
Organism Escherichia coli strain BR1220

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag MM431_RS19570 Protein ID WP_000615983.1
Coordinates 3988683..3988961 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag MM431_RS19565 Protein ID WP_000729703.1
Coordinates 3988420..3988680 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM431_RS19550 (3984471) 3984471..3986564 + 2094 WP_061357700.1 flagellar biosynthesis protein FlhA -
MM431_RS19555 (3986788) 3986788..3987285 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
MM431_RS19560 (3987461) 3987461..3988210 - 750 WP_000056849.1 C40 family peptidase -
MM431_RS19565 (3988420) 3988420..3988680 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MM431_RS19570 (3988683) 3988683..3988961 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MM431_RS19575 (3989117) 3989117..3989857 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MM431_RS19580 (3989828) 3989828..3990595 - 768 WP_000333380.1 class II glutamine amidotransferase -
MM431_RS19585 (3990801) 3990801..3991379 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / clpV/tssH / tssA / tssA / hcp1/tssD1 3987461..4014206 26745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T237682 WP_000615983.1 NZ_CP093068:3988683-3988961 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T237682 NZ_LR882499:c2206919-2206590 [Mycobacterium tuberculosis variant microti OV254]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT237682 WP_000729703.1 NZ_CP093068:3988420-3988680 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT237682 NZ_LR882499:c2207293-2206916 [Mycobacterium tuberculosis variant microti OV254]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References