Detailed information of TA system TA237681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3946641..3947335
• Replicon: chromosome
• Accession: NZ_CP093068
• Organism: Escherichia coli strain BR1220

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: MM431_RS19330
• Protein ID: WP_001263489.1
• Coordinates: 3946641..3947039 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MM431_RS19335
• Protein ID: WP_000554758.1
• Coordinates: 3947042..3947335 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3942229)	3942229..3942309	-	81	NuclAT_12	-	-
- (3942229)	3942229..3942309	-	81	NuclAT_12	-	-
- (3942229)	3942229..3942309	-	81	NuclAT_12	-	-
- (3942229)	3942229..3942309	-	81	NuclAT_12	-	-
MM431_RS19305 (3942905)	3942905..3943363	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
MM431_RS19310 (3943624)	3943624..3945081	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
MM431_RS19315 (3945138)	3945138..3945659	-	522	Protein_3803	peptide chain release factor H	-
MM431_RS19320 (3945655)	3945655..3945861	-	207	Protein_3804	RtcB family protein	-
MM431_RS19325 (3946179)	3946179..3946631	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
MM431_RS19330 (3946641)	3946641..3947039	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MM431_RS19335 (3947042)	3947042..3947335	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MM431_RS19340 (3947387)	3947387..3948442	-	1056	WP_001226164.1	DNA polymerase IV	-
MM431_RS19345 (3948513)	3948513..3949436	-	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
MM431_RS19350 (3949439)	3949439..3950302	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
MM431_RS19355 (3950315)	3950315..3951031	-	717	Protein_3811	FliA/WhiG family RNA polymerase sigma factor	-
MM431_RS19360 (3951051)	3951051..3951518	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T237681 WP_001263489.1 NZ_CP093068:c3947039-3946641 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T237681 NZ_LR882499:1968632-1968880 [Mycobacterium tuberculosis variant microti OV254]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT237681 WP_000554758.1 NZ_CP093068:c3947335-3947042 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT237681 NZ_LR882499:1968420-1968632 [Mycobacterium tuberculosis variant microti OV254]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3