Detailed information of TA system TA237610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5985120..5985715
• Replicon: chromosome
• Accession: NZ_CP093032
• Organism: Pseudomonas aeruginosa strain H02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MMZ65_RS28105
• Protein ID: WP_003113526.1
• Coordinates: 5985437..5985715 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMZ65_RS28100
• Protein ID: WP_003133769.1
• Coordinates: 5985120..5985425 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ65_RS28070 (MMZ65_28070)	5980490..5980762	+	273	WP_071538125.1	DNA-binding protein	-
MMZ65_RS28075 (MMZ65_28075)	5980820..5981401	+	582	WP_123903235.1	sce7726 family protein	-
MMZ65_RS28080 (MMZ65_28080)	5981388..5982458	-	1071	WP_023098928.1	beta family protein	-
MMZ65_RS28085 (MMZ65_28085)	5982448..5983284	-	837	WP_023098927.1	ImmA/IrrE family metallo-endopeptidase	-
MMZ65_RS28090 (MMZ65_28090)	5983277..5983951	-	675	WP_023098926.1	N-acetyltransferase	-
MMZ65_RS28095 (MMZ65_28095)	5984377..5984784	-	408	WP_049267485.1	hypothetical protein	-
MMZ65_RS28100 (MMZ65_28100)	5985120..5985425	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
MMZ65_RS28105 (MMZ65_28105)	5985437..5985715	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ65_RS28110 (MMZ65_28110)	5985768..5985896	-	129	Protein_5564	integrase	-
MMZ65_RS28115 (MMZ65_28115)	5986044..5988272	+	2229	WP_003141628.1	TonB-dependent receptor	-
MMZ65_RS28120 (MMZ65_28120)	5988342..5988989	-	648	WP_003095021.1	carbonate dehydratase	-
MMZ65_RS28125 (MMZ65_28125)	5989051..5990289	-	1239	WP_003141631.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T237610 WP_003113526.1 NZ_CP093032:c5985715-5985437 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237610 NZ_LR882498:c757788-757351 [Mycobacterium tuberculosis variant microti]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT237610 WP_003133769.1 NZ_CP093032:c5985425-5985120 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237610 NZ_LR882498:c758039-757785 [Mycobacterium tuberculosis variant microti]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Toxin

• AlphaFold DB: V6ALY3