Detailed information of TA system TA237604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5844748..5845343
• Replicon: chromosome
• Accession: NZ_CP093031
• Organism: Pseudomonas aeruginosa strain H03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MMZ66_RS27500
• Protein ID: WP_003113526.1
• Coordinates: 5845065..5845343 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMZ66_RS27495
• Protein ID: WP_003133769.1
• Coordinates: 5844748..5845053 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ66_RS27480 (MMZ66_27490)	5841470..5842333	-	864	WP_023098850.1	integrase domain-containing protein	-
MMZ66_RS27485 (MMZ66_27495)	5842934..5844076	-	1143	WP_023098851.1	STY4528 family pathogenicity island replication protein	-
MMZ66_RS27495 (MMZ66_27505)	5844748..5845053	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
MMZ66_RS27500 (MMZ66_27510)	5845065..5845343	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ66_RS27505 (MMZ66_27515)	5845396..5845524	-	129	Protein_5443	integrase	-
MMZ66_RS27510 (MMZ66_27520)	5845672..5847900	+	2229	WP_023104079.1	TonB-dependent receptor	-
MMZ66_RS27515 (MMZ66_27525)	5847971..5848618	-	648	WP_003095021.1	carbonate dehydratase	-
MMZ66_RS27520 (MMZ66_27530)	5848680..5849918	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T237604 WP_003113526.1 NZ_CP093031:c5845343-5845065 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237604 NZ_LR882498:712488-712889 [Mycobacterium tuberculosis variant microti]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT237604 WP_003133769.1 NZ_CP093031:c5845053-5844748 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237604 NZ_LR882498:712264-712491 [Mycobacterium tuberculosis variant microti]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGTCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3