Detailed information of TA system TA237588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5968937..5969532
• Replicon: chromosome
• Accession: NZ_CP093028
• Organism: Pseudomonas aeruginosa strain H05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MMZ68_RS28110
• Protein ID: WP_003113526.1
• Coordinates: 5969254..5969532 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMZ68_RS28105
• Protein ID: WP_003133769.1
• Coordinates: 5968937..5969242 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ68_RS28070 (MMZ68_28070)	5964078..5964926	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MMZ68_RS28080 (MMZ68_28080)	5965093..5966034	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MMZ68_RS28085 (MMZ68_28085)	5966151..5966765	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
MMZ68_RS28090 (MMZ68_28090)	5966807..5967391	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MMZ68_RS28095 (MMZ68_28095)	5967432..5968532	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MMZ68_RS28105 (MMZ68_28105)	5968937..5969242	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
MMZ68_RS28110 (MMZ68_28110)	5969254..5969532	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ68_RS28115 (MMZ68_28115)	5969585..5969713	-	129	Protein_5571	integrase	-
MMZ68_RS28120 (MMZ68_28120)	5969861..5972089	+	2229	WP_019485341.1	TonB-dependent receptor	-
MMZ68_RS28125 (MMZ68_28125)	5972159..5972806	-	648	WP_003095021.1	carbonate dehydratase	-
MMZ68_RS28130 (MMZ68_28130)	5972868..5974106	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T237588 WP_003113526.1 NZ_CP093028:c5969532-5969254 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237588 NZ_LR882497:3177713-3177976 [Mycobacterium tuberculosis variant microti]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT237588 WP_003133769.1 NZ_CP093028:c5969242-5968937 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237588 NZ_LR882497:3177428-3177709 [Mycobacterium tuberculosis variant microti]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• AlphaFold DB: V6ALY3