Detailed information of TA system TA237580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5481157..5481752
• Replicon: chromosome
• Accession: NZ_CP093023
• Organism: Pseudomonas aeruginosa strain H07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MMZ70_RS25430
• Protein ID: WP_003113526.1
• Coordinates: 5481474..5481752 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMZ70_RS25425
• Protein ID: WP_003099268.1
• Coordinates: 5481157..5481462 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ70_RS25390 (MMZ70_25395)	5476904..5477548	-	645	WP_104052955.1	DNA cytosine methyltransferase	-
MMZ70_RS25395 (MMZ70_25400)	5477556..5477843	-	288	WP_104052957.1	DUF5447 family protein	-
MMZ70_RS25400 (MMZ70_25405)	5477847..5478062	-	216	WP_104052958.1	hypothetical protein	-
MMZ70_RS25405 (MMZ70_25410)	5478055..5478327	-	273	WP_003115921.1	hypothetical protein	-
MMZ70_RS25410 (MMZ70_25415)	5478774..5479112	+	339	WP_031629305.1	hypothetical protein	-
MMZ70_RS25415 (MMZ70_25420)	5479117..5480187	-	1071	WP_023110436.1	reverse transcriptase family protein	-
MMZ70_RS25420 (MMZ70_25425)	5480263..5480481	-	219	WP_031629306.1	hypothetical protein	-
MMZ70_RS25425 (MMZ70_25430)	5481157..5481462	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
MMZ70_RS25430 (MMZ70_25435)	5481474..5481752	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ70_RS25435 (MMZ70_25440)	5481805..5481933	-	129	Protein_5034	integrase	-
MMZ70_RS25440 (MMZ70_25445)	5482081..5484309	+	2229	WP_031633241.1	TonB-dependent receptor	-
MMZ70_RS25445 (MMZ70_25450)	5484379..5485026	-	648	WP_003095021.1	carbonate dehydratase	-
MMZ70_RS25450 (MMZ70_25455)	5485088..5486326	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T237580 WP_003113526.1 NZ_CP093023:c5481752-5481474 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237580 NZ_CP093023:c5481752-5481474 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT237580 WP_003099268.1 NZ_CP093023:c5481462-5481157 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237580 NZ_CP093023:c5481462-5481157 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3