Detailed information of TA system TA237575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5786866..5787461
• Replicon: chromosome
• Accession: NZ_CP093022
• Organism: Pseudomonas aeruginosa strain H08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MMZ71_RS27340
• Protein ID: WP_071535843.1
• Coordinates: 5787183..5787461 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: MMZ71_RS27335
• Protein ID: WP_003111575.1
• Coordinates: 5786866..5787171 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ71_RS27300 (MMZ71_27305)	5782006..5782854	+	849	WP_009316211.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MMZ71_RS27310 (MMZ71_27315)	5783021..5783962	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MMZ71_RS27315 (MMZ71_27320)	5784079..5784693	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
MMZ71_RS27320 (MMZ71_27325)	5784735..5785319	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MMZ71_RS27325 (MMZ71_27330)	5785360..5786460	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MMZ71_RS27335 (MMZ71_27340)	5786866..5787171	-	306	WP_003111575.1	HigA family addiction module antitoxin	Antitoxin
MMZ71_RS27340 (MMZ71_27345)	5787183..5787461	-	279	WP_071535843.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ71_RS27345 (MMZ71_27350)	5787514..5787642	-	129	Protein_5414	integrase	-
MMZ71_RS27350 (MMZ71_27355)	5787790..5790018	+	2229	WP_021264080.1	TonB-dependent receptor	-
MMZ71_RS27355 (MMZ71_27360)	5790088..5790735	-	648	WP_003095021.1	carbonate dehydratase	-
MMZ71_RS27360 (MMZ71_27365)	5790797..5792035	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10674.25 Da
• Isoelectric Point: 7.8937

>T237575 WP_071535843.1 NZ_CP093022:c5787461-5787183 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRVGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237575 NZ_LR882497:c2409003-2408686 [Mycobacterium tuberculosis variant microti]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT237575 WP_003111575.1 NZ_CP093022:c5787171-5786866 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT237575 NZ_LR882497:c2409215-2409000 [Mycobacterium tuberculosis variant microti]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4