Detailed information of TA system TA237573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4937139..4937820
• Replicon: chromosome
• Accession: NZ_CP093022
• Organism: Pseudomonas aeruginosa strain H08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: MMZ71_RS23190
• Protein ID: WP_003111825.1
• Coordinates: 4937455..4937820 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMZ71_RS23185
• Protein ID: WP_003145733.1
• Coordinates: 4937139..4937462 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ71_RS23160 (MMZ71_23165)	4933052..4933690	+	639	WP_003163192.1	hypothetical protein	-
MMZ71_RS23165 (MMZ71_23170)	4933933..4934268	+	336	WP_003140884.1	TM2 domain-containing protein	-
MMZ71_RS23170 (MMZ71_23175)	4934442..4934960	+	519	WP_022579807.1	PAAR domain-containing protein	-
MMZ71_RS23175 (MMZ71_23180)	4934957..4935658	+	702	WP_033954262.1	VRR-NUC domain-containing protein	-
MMZ71_RS23180 (MMZ71_23185)	4935675..4936763	+	1089	WP_009315279.1	DUF3396 domain-containing protein	-
MMZ71_RS23185 (MMZ71_23190)	4937139..4937462	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
MMZ71_RS23190 (MMZ71_23195)	4937455..4937820	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ71_RS23195 (MMZ71_23200)	4938084..4938323	-	240	WP_044060930.1	hypothetical protein	-
MMZ71_RS23200 (MMZ71_23205)	4938530..4938802	+	273	WP_033954259.1	hypothetical protein	-
MMZ71_RS23205 (MMZ71_23210)	4938833..4939258	-	426	WP_003116492.1	VOC family protein	-
MMZ71_RS23210 (MMZ71_23215)	4939359..4940243	+	885	WP_033939105.1	LysR substrate-binding domain-containing protein	-
MMZ71_RS23215 (MMZ71_23220)	4940216..4941169	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
MMZ71_RS23220 (MMZ71_23225)	4941390..4941824	+	435	WP_003114204.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4923259..4940243	16984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T237573 WP_003111825.1 NZ_CP093022:c4937820-4937455 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T237573 NZ_LR882497:2326895-2327305 [Mycobacterium tuberculosis variant microti]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT237573 WP_003145733.1 NZ_CP093022:c4937462-4937139 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT237573 NZ_LR882497:2326669-2326902 [Mycobacterium tuberculosis variant microti]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: V6AKP0