Detailed information of TA system TA237562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5639131..5639726
• Replicon: chromosome
• Accession: NZ_CP093020
• Organism: Pseudomonas aeruginosa strain H10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MMZ73_RS26595
• Protein ID: WP_003117425.1
• Coordinates: 5639448..5639726 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMZ73_RS26590
• Protein ID: WP_003113527.1
• Coordinates: 5639131..5639436 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ73_RS26555 (MMZ73_26570)	5634270..5635118	+	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MMZ73_RS26565 (MMZ73_26580)	5635285..5636226	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MMZ73_RS26570 (MMZ73_26585)	5636343..5636957	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
MMZ73_RS26575 (MMZ73_26590)	5636999..5637583	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MMZ73_RS26580 (MMZ73_26595)	5637624..5638724	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MMZ73_RS26590 (MMZ73_26605)	5639131..5639436	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MMZ73_RS26595 (MMZ73_26610)	5639448..5639726	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMZ73_RS26600 (MMZ73_26615)	5639779..5639907	-	129	Protein_5263	integrase	-
MMZ73_RS26605 (MMZ73_26620)	5640055..5642283	+	2229	WP_003113525.1	TonB-dependent receptor	-
MMZ73_RS26610 (MMZ73_26625)	5642353..5643000	-	648	WP_003095021.1	carbonate dehydratase	-
MMZ73_RS26615 (MMZ73_26630)	5643062..5644300	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T237562 WP_003117425.1 NZ_CP093020:c5639726-5639448 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237562 NZ_LR882497:1696554-1696871 [Mycobacterium tuberculosis variant microti]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT237562 WP_003113527.1 NZ_CP093020:c5639436-5639131 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237562 NZ_LR882497:1696255-1696557 [Mycobacterium tuberculosis variant microti]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5