Detailed information of TA system TA237531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5297272..5297867
• Replicon: chromosome
• Accession: NZ_CP093012
• Organism: Pseudomonas aeruginosa strain H20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MM141_RS24695
• Protein ID: WP_003113526.1
• Coordinates: 5297589..5297867 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MM141_RS24690
• Protein ID: WP_003113527.1
• Coordinates: 5297272..5297577 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM141_RS24660 (MM141_24670)	5292375..5292665	-	291	WP_057387714.1	DUF5447 family protein	-
MM141_RS24665 (MM141_24675)	5292877..5293149	-	273	WP_003115921.1	hypothetical protein	-
MM141_RS24670 (MM141_24680)	5293259..5293525	+	267	WP_023088595.1	hypothetical protein	-
MM141_RS24675 (MM141_24685)	5293581..5293919	+	339	WP_079385463.1	hypothetical protein	-
MM141_RS24680 (MM141_24690)	5294012..5296507	+	2496	WP_126475989.1	hypothetical protein	-
MM141_RS24685 (MM141_24695)	5296549..5296857	-	309	WP_126475988.1	hypothetical protein	-
MM141_RS24690 (MM141_24700)	5297272..5297577	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MM141_RS24695 (MM141_24705)	5297589..5297867	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MM141_RS24700 (MM141_24710)	5297920..5298048	-	129	Protein_4880	integrase	-
MM141_RS24705 (MM141_24715)	5298196..5300424	+	2229	WP_003113525.1	TonB-dependent receptor	-
MM141_RS24710 (MM141_24720)	5300494..5301141	-	648	WP_003095021.1	carbonate dehydratase	-
MM141_RS24715 (MM141_24725)	5301203..5302441	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T237531 WP_003113526.1 NZ_CP093012:c5297867-5297589 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237531 NZ_LR882496:3172544-3172987 [Mycobacterium tuberculosis variant microti]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT237531 WP_003113527.1 NZ_CP093012:c5297577-5297272 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237531 NZ_LR882496:3172300-3172557 [Mycobacterium tuberculosis variant microti]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: V6ALY3