Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3781211..3781713 Replicon chromosome
Accession NZ_CP093011
Organism Escherichia coli strain 131

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag LJF86_RS18150 Protein ID WP_000767822.1
Coordinates 3781459..3781713 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LJF86_RS18145 Protein ID WP_001259255.1
Coordinates 3781211..3781462 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF86_RS18120 (3776389) 3776389..3777456 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
LJF86_RS18125 (3777456) 3777456..3778526 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
LJF86_RS18130 (3778523) 3778523..3779827 - 1305 WP_001546022.1 histidinol dehydrogenase -
LJF86_RS18135 (3779833) 3779833..3780732 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
LJF86_RS18140 (3780878) 3780878..3780928 - 51 WP_001364200.1 his operon leader peptide -
LJF86_RS18145 (3781211) 3781211..3781462 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LJF86_RS18150 (3781459) 3781459..3781713 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LJF86_RS18155 (3781796) 3781796..3782620 + 825 WP_000754737.1 SDR family oxidoreductase -
LJF86_RS18160 (3782666) 3782666..3783595 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
LJF86_RS18165 (3783810) 3783810..3783872 + 63 WP_010723108.1 membrane protein YoeI -
LJF86_RS18170 (3783862) 3783862..3785220 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T237510 WP_000767822.1 NZ_CP093011:3781459-3781713 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T237510 NZ_LR882496:c2209016-2208720 [Mycobacterium tuberculosis variant microti]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT237510 WP_001259255.1 NZ_CP093011:3781211-3781462 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT237510 NZ_LR882496:c2209264-2209013 [Mycobacterium tuberculosis variant microti]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References