Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1542230..1542957 Replicon chromosome
Accession NZ_CP093011
Organism Escherichia coli strain 131

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LJF86_RS07700 Protein ID WP_000550189.1
Coordinates 1542230..1542544 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LJF86_RS07705 Protein ID WP_000560269.1
Coordinates 1542541..1542957 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF86_RS07680 (1538387) 1538387..1539373 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
LJF86_RS07685 (1539452) 1539452..1540144 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
LJF86_RS07690 (1540221) 1540221..1540724 - 504 WP_001295542.1 M48 family metallopeptidase -
LJF86_RS07695 (1540809) 1540809..1541945 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LJF86_RS07700 (1542230) 1542230..1542544 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LJF86_RS07705 (1542541) 1542541..1542957 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LJF86_RS07710 (1543002) 1543002..1545020 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LJF86_RS07715 (1545246) 1545246..1547597 - 2352 WP_000695431.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T237503 WP_000550189.1 NZ_CP093011:1542230-1542544 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T237503 NZ_LR882496:c1582280-1581879 [Mycobacterium tuberculosis variant microti]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT237503 WP_000560269.1 NZ_CP093011:1542541-1542957 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT237503 NZ_LR882496:c1582534-1582277 [Mycobacterium tuberculosis variant microti]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References