Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1108325..1109004 Replicon chromosome
Accession NZ_CP093011
Organism Escherichia coli strain 131

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag LJF86_RS05570 Protein ID WP_000057523.1
Coordinates 1108702..1109004 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LJF86_RS05565 Protein ID WP_000806442.1
Coordinates 1108325..1108666 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF86_RS05555 (1104569) 1104569..1105501 - 933 WP_000883041.1 glutaminase A -
LJF86_RS05560 (1105763) 1105763..1108267 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
LJF86_RS05565 (1108325) 1108325..1108666 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LJF86_RS05570 (1108702) 1108702..1109004 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LJF86_RS05575 (1109137) 1109137..1109931 + 795 WP_000365147.1 TraB/GumN family protein -
LJF86_RS05580 (1110135) 1110135..1110614 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LJF86_RS05585 (1110782) 1110782..1111438 + 657 WP_015674862.1 hypothetical protein -
LJF86_RS05590 (1111435) 1111435..1111938 + 504 WP_000667000.1 hypothetical protein -
LJF86_RS05595 (1111976) 1111976..1113628 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T237499 WP_000057523.1 NZ_CP093011:c1109004-1108702 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T237499 NZ_LR882496:1242760-1243134 [Mycobacterium tuberculosis variant microti]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT237499 WP_000806442.1 NZ_CP093011:c1108666-1108325 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT237499 NZ_LR882496:1242566-1242763 [Mycobacterium tuberculosis variant microti]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References