Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40264..40914 Replicon chromosome
Accession NZ_CP093010
Organism Klebsiella michiganensis strain K342

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag MMY94_RS00195 Protein ID WP_014227314.1
Coordinates 40264..40605 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag MMY94_RS00200 Protein ID WP_014227313.1
Coordinates 40615..40914 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY94_RS00180 (MMY94_00180) 36527..37846 + 1320 WP_032694296.1 MFS transporter -
MMY94_RS00185 (MMY94_00185) 37992..39383 + 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
MMY94_RS00190 (MMY94_00190) 39561..40013 + 453 WP_009653860.1 DUF1198 domain-containing protein -
MMY94_RS00195 (MMY94_00195) 40264..40605 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MMY94_RS00200 (MMY94_00200) 40615..40914 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
MMY94_RS00205 (MMY94_00205) 41033..42214 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
MMY94_RS00210 (MMY94_00210) 42247..43056 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
MMY94_RS00215 (MMY94_00215) 43066..44169 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
MMY94_RS00220 (MMY94_00220) 44310..45029 + 720 WP_032694297.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T237473 WP_014227314.1 NZ_CP093010:40264-40605 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T237473 NZ_LR882493:c4246799-4246449 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT237473 WP_014227313.1 NZ_CP093010:40615-40914 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT237473 NZ_LR882493:c4247044-4246793 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References