Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3758467..3759008 Replicon chromosome
Accession NZ_CP092973
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag MM176_RS18155 Protein ID WP_000615983.1
Coordinates 3758730..3759008 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag MM176_RS18150 Protein ID WP_000729703.1
Coordinates 3758467..3758727 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM176_RS18130 (3754142) 3754142..3754927 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MM176_RS18135 (3754899) 3754899..3756611 + 1713 Protein_3573 flagellar biosynthesis protein FlhA -
MM176_RS18140 (3756835) 3756835..3757332 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
MM176_RS18145 (3757508) 3757508..3758257 - 750 WP_000056849.1 C40 family peptidase -
MM176_RS18150 (3758467) 3758467..3758727 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MM176_RS18155 (3758730) 3758730..3759008 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MM176_RS18160 (3759164) 3759164..3759904 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MM176_RS18165 (3759875) 3759875..3760642 - 768 WP_000333380.1 class II glutamine amidotransferase -
MM176_RS18170 (3760848) 3760848..3761426 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T237456 WP_000615983.1 NZ_CP092973:3758730-3759008 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T237456 NZ_LR882061:21831-21989 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT237456 WP_000729703.1 NZ_CP092973:3758467-3758727 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT237456 NZ_LR882061:21720-21785 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References