Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3752270..3752964 Replicon chromosome
Accession NZ_CP092973
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag MM176_RS18115 Protein ID WP_001263489.1
Coordinates 3752270..3752668 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MM176_RS18120 Protein ID WP_000554758.1
Coordinates 3752671..3752964 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3747858) 3747858..3747938 - 81 NuclAT_11 - -
- (3747858) 3747858..3747938 - 81 NuclAT_11 - -
- (3747858) 3747858..3747938 - 81 NuclAT_11 - -
- (3747858) 3747858..3747938 - 81 NuclAT_11 - -
MM176_RS18090 (3748534) 3748534..3748992 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
MM176_RS18095 (3749253) 3749253..3750710 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
MM176_RS18100 (3750767) 3750767..3751288 - 522 Protein_3566 peptide chain release factor H -
MM176_RS18105 (3751284) 3751284..3751490 - 207 Protein_3567 RtcB family protein -
MM176_RS18110 (3751808) 3751808..3752260 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
MM176_RS18115 (3752270) 3752270..3752668 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MM176_RS18120 (3752671) 3752671..3752964 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MM176_RS18125 (3753016) 3753016..3754071 - 1056 WP_001226164.1 DNA polymerase IV -
MM176_RS18130 (3754142) 3754142..3754927 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MM176_RS18135 (3754899) 3754899..3756611 + 1713 Protein_3573 flagellar biosynthesis protein FlhA -
MM176_RS18140 (3756835) 3756835..3757332 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T237455 WP_001263489.1 NZ_CP092973:c3752668-3752270 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T237455 NZ_LR882060:c46384-46103 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT237455 WP_000554758.1 NZ_CP092973:c3752964-3752671 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT237455 NZ_LR882060:c46625-46374 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References