Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612170..612969 Replicon chromosome
Accession NZ_CP092973
Organism Escherichia coli strain WCHEC005215

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag MM176_RS02980 Protein ID WP_000347275.1
Coordinates 612170..612634 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MM176_RS02985 Protein ID WP_001307405.1
Coordinates 612634..612969 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MM176_RS02950 (607171) 607171..607605 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MM176_RS02955 (607623) 607623..608501 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MM176_RS02960 (608491) 608491..609270 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MM176_RS02965 (609281) 609281..609754 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MM176_RS02970 (609777) 609777..611057 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MM176_RS02975 (611306) 611306..612115 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MM176_RS02980 (612170) 612170..612634 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MM176_RS02985 (612634) 612634..612969 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MM176_RS02990 (613118) 613118..614689 - 1572 WP_001273752.1 galactarate dehydratase -
MM176_RS02995 (615064) 615064..616398 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MM176_RS03000 (616414) 616414..617184 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T237436 WP_000347275.1 NZ_CP092973:c612634-612170 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T237436 NZ_LR882057:1065110-1065445 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT237436 WP_001307405.1 NZ_CP092973:c612969-612634 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT237436 NZ_LR882057:1064862-1065110 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References