Detailed information of TA system TA237435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5230310..5230905
• Replicon: chromosome
• Accession: NZ_CP092972
• Organism: Pseudomonas aeruginosa strain PA01135

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MMG97_RS24260
• Protein ID: WP_003113526.1
• Coordinates: 5230627..5230905 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMG97_RS24255
• Protein ID: WP_003113527.1
• Coordinates: 5230310..5230615 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMG97_RS24220 (MMG97_24180)	5225449..5226297	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MMG97_RS24230 (MMG97_24190)	5226464..5227405	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MMG97_RS24235 (MMG97_24195)	5227522..5228136	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
MMG97_RS24240 (MMG97_24200)	5228178..5228762	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MMG97_RS24245 (MMG97_24205)	5228803..5229903	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MMG97_RS24255 (MMG97_24215)	5230310..5230615	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MMG97_RS24260 (MMG97_24220)	5230627..5230905	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MMG97_RS24265 (MMG97_24225)	5230958..5231086	-	129	Protein_4791	integrase	-
MMG97_RS24270 (MMG97_24230)	5231234..5233462	+	2229	WP_003113525.1	TonB-dependent receptor	-
MMG97_RS24275 (MMG97_24235)	5233532..5234179	-	648	WP_003095021.1	carbonate dehydratase	-
MMG97_RS24280 (MMG97_24240)	5234241..5235479	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T237435 WP_003113526.1 NZ_CP092972:c5230905-5230627 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T237435 NZ_LR882057:939420-939827 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT237435 WP_003113527.1 NZ_CP092972:c5230615-5230310 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT237435 NZ_LR882057:939173-939439 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3