Detailed information of TA system TA237394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2507658..2508385
• Replicon: chromosome
• Accession: NZ_CP092911
• Organism: Salmonella enterica subsp. enterica strain SR33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8E5IJ81
• Locus tag: MLD58_RS12265
• Protein ID: WP_000558157.1
• Coordinates: 2507658..2507969 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MLD58_RS12270
• Protein ID: WP_000561389.1
• Coordinates: 2507966..2508385 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLD58_RS12225 (2502702)	2502702..2503097	+	396	WP_000422887.1	DUF1398 domain-containing protein	-
MLD58_RS12230 (2503426)	2503426..2503902	+	477	WP_023138823.1	hypothetical protein	-
MLD58_RS12235 (2504054)	2504054..2504206	+	153	WP_157794946.1	hypothetical protein	-
MLD58_RS12240 (2504318)	2504318..2504776	-	459	WP_001195655.1	hypothetical protein	-
MLD58_RS12245 (2505102)	2505102..2506249	+	1148	WP_112324583.1	IS3 family transposase	-
MLD58_RS12250 (2506280)	2506280..2506837	-	558	WP_000179886.1	hypothetical protein	-
MLD58_RS12255 (2506941)	2506941..2507150	-	210	WP_023137948.1	hypothetical protein	-
MLD58_RS12260 (2507180)	2507180..2507479	-	300	WP_000775225.1	hypothetical protein	-
MLD58_RS12265 (2507658)	2507658..2507969	+	312	WP_000558157.1	type II toxin-antitoxin system HigB family toxin	Toxin
MLD58_RS12270 (2507966)	2507966..2508385	+	420	WP_000561389.1	helix-turn-helix domain-containing protein	Antitoxin
MLD58_RS12275 (2508638)	2508638..2509290	+	653	Protein_2401	glycosyltransferase	-
MLD58_RS12280 (2509526)	2509526..2509945	-	420	WP_023137951.1	GNAT family N-acetyltransferase	-
MLD58_RS12285 (2510315)	2510315..2510584	+	270	WP_077908593.1	hypothetical protein	-
MLD58_RS12290 (2510750)	2510750..2510890	+	141	WP_001576018.1	hypothetical protein	-
MLD58_RS12295 (2511036)	2511036..2511577	-	542	Protein_2405	transposase	-
MLD58_RS12300 (2511597)	2511597..2511689	-	93	WP_231923105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2505102..2511374	6272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12425.26 Da
• Isoelectric Point: 10.1376

>T237394 WP_000558157.1 NZ_CP092911:2507658-2507969 [Salmonella enterica subsp. enterica]
MHVISKEPFDEAARRYPNDSLAIKALYRLVREKDFSSPAELRKVIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFINKRF
YVKHIATHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T237394 NZ_LR882050:3295407-3295514 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15301.49 Da
• Isoelectric Point: 4.6286

>AT237394 WP_000561389.1 NZ_CP092911:2507966-2508385 [Salmonella enterica subsp. enterica]
MIANTAKAIEATKALVAAVPFLGGSASEKDYRDALALVDYLIENDDENPLIDFLASKIAEYEDNSKQFAEFNKSVAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT237394 NZ_LR882050:c3295350-3295295 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT