Detailed information of TA system TA237298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4906243..4907003
• Replicon: chromosome
• Accession: NZ_CP092860
• Organism: Citrobacter braakii strain P1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: MJ613_RS23945
• Protein ID: WP_019077936.1
• Coordinates: 4906243..4906554 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ613_RS23950
• Protein ID: WP_104651900.1
• Coordinates: 4906551..4907003 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ613_RS23915 (MJ613_23905)	4901910..4902812	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
MJ613_RS23920 (MJ613_23910)	4902809..4903444	+	636	WP_185655067.1	formate dehydrogenase cytochrome b556 subunit	-
MJ613_RS23925 (MJ613_23915)	4903441..4904370	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
MJ613_RS23930 (MJ613_23920)	4904407..4904781	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
MJ613_RS23935 (MJ613_23925)	4904781..4905023	-	243	WP_016155181.1	CopG family transcriptional regulator	-
MJ613_RS23940 (MJ613_23930)	4905229..4906158	+	930	WP_185655066.1	alpha/beta hydrolase	-
MJ613_RS23945 (MJ613_23935)	4906243..4906554	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
MJ613_RS23950 (MJ613_23940)	4906551..4907003	+	453	WP_104651900.1	helix-turn-helix domain-containing protein	Antitoxin
MJ613_RS23955 (MJ613_23945)	4907021..4907962	-	942	WP_278085725.1	fatty acid biosynthesis protein FabY	-
MJ613_RS23960 (MJ613_23950)	4908007..4908444	-	438	WP_016157874.1	D-aminoacyl-tRNA deacylase	-
MJ613_RS23965 (MJ613_23955)	4908441..4909313	-	873	WP_019077938.1	virulence factor BrkB family protein	-
MJ613_RS23970 (MJ613_23960)	4909307..4909906	-	600	WP_016155174.1	glucose-1-phosphatase	-
MJ613_RS23975 (MJ613_23965)	4910011..4910814	-	804	WP_016155173.1	DeoR/GlpR family DNA-binding transcription regulator	-
MJ613_RS23980 (MJ613_23970)	4910848..4911744	-	897	WP_019077939.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T237298 WP_019077936.1 NZ_CP092860:4906243-4906554 [Citrobacter braakii]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T237298 NZ_LR878366:c137270-136965 [Shigella flexneri 3a]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17028.41 Da
• Isoelectric Point: 5.9259

>AT237298 WP_104651900.1 NZ_CP092860:4906551-4907003 [Citrobacter braakii]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVTLLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT237298 NZ_LR878366:c137490-137272 [Shigella flexneri 3a]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAATAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7L6U900