Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 43317..43966 Replicon chromosome
Accession NZ_CP092860
Organism Citrobacter braakii strain P1

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6TVT2
Locus tag MJ613_RS00205 Protein ID WP_016157800.1
Coordinates 43317..43658 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7L6TWH1
Locus tag MJ613_RS00210 Protein ID WP_016157799.1
Coordinates 43667..43966 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ613_RS00190 (MJ613_00190) 39753..41081 + 1329 WP_278085730.1 MFS transporter -
MJ613_RS00195 (MJ613_00195) 41224..42615 + 1392 WP_185654897.1 hexose-6-phosphate:phosphate antiporter -
MJ613_RS00200 (MJ613_00200) 42764..43216 + 453 WP_016157801.1 DUF1198 family protein -
MJ613_RS00205 (MJ613_00205) 43317..43658 + 342 WP_016157800.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ613_RS00210 (MJ613_00210) 43667..43966 + 300 WP_016157799.1 helix-turn-helix transcriptional regulator Antitoxin
MJ613_RS00215 (MJ613_00215) 44086..45270 + 1185 WP_016155040.1 purine ribonucleoside efflux pump NepI -
MJ613_RS00220 (MJ613_00220) 45326..46708 - 1383 WP_047415720.1 glycoside hydrolase family 1 protein -
MJ613_RS00225 (MJ613_00225) 46801..47094 - 294 WP_019078023.1 YicS family protein -
MJ613_RS00230 (MJ613_00230) 47225..47641 + 417 WP_181633089.1 GNAT family N-acetyltransferase -
MJ613_RS00235 (MJ613_00235) 47648..47791 + 144 Protein_46 ISNCY family transposase -
MJ613_RS00240 (MJ613_00240) 47917..48735 + 819 WP_016157795.1 lipoprotein NlpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13061.92 Da        Isoelectric Point: 5.6550

>T237285 WP_016157800.1 NZ_CP092860:43317-43658 [Citrobacter braakii]
MWEVETTDVFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGNPIRAFFVFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLNK

Download         Length: 342 bp

>T237285 NZ_LR878365:c1650110-1649955 [Shigella flexneri 3a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10988.84 Da        Isoelectric Point: 8.6007

>AT237285 WP_016157799.1 NZ_CP092860:43667-43966 [Citrobacter braakii]
MATLKELMAKQSPESQERIAEKVEVLRQVIALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Download         Length: 300 bp

>AT237285 NZ_LR878365:1650122-1650180 [Shigella flexneri 3a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6TVT2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6TWH1

References