Detailed information of TA system TA023726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 353377..353992
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: VC395_RS15870
• Protein ID: WP_001162670.1
• Coordinates: 353377..353664 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VC395_RS15875
• Protein ID: WP_001232701.1
• Coordinates: 353675..353992 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS21030	349013..349108	+	96	WP_012706049.1	DUF645 family protein	-
VC395_RS15835	349302..349619	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC395_RS15840	349638..349880	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VC395_RS15845	350122..350631	+	510	WP_000405986.1	GNAT family N-acetyltransferase	-
VC395_RS15850	350688..351341	+	654	WP_000226874.1	hypothetical protein	-
VC395_RS15855	351651..351869	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
VC395_RS15860	352272..352505	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
VC395_RS15865	352930..353110	+	181	Protein_313	DUF645 family protein	-
VC395_RS15870	353377..353664	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC395_RS15875	353675..353992	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
VC395_RS21035	353989..354099	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
VC395_RS20120	354276..354401	+	126	WP_001944767.1	DUF645 family protein	-
VC395_RS20805	354417..354455	+	39	WP_106019119.1	hypothetical protein	-
VC395_RS15885	354679..355419	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
VC395_RS15890	355624..356454	+	831	WP_000056616.1	abortive infection family protein	-
VC395_RS15895	356511..356993	+	483	WP_001895003.1	hypothetical protein	-
VC395_RS15905	357440..358315	+	876	WP_000259044.1	hypothetical protein	-
VC395_RS15910	358445..358900	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	344619..430484	85865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T23726 WP_001162670.1 NC_012583:353377-353664 [Vibrio cholerae O395]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T23726 NC_012583:353377-353664 [Vibrio cholerae O395]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT23726 WP_001232701.1 NC_012583:353675-353992 [Vibrio cholerae O395]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT23726 NC_012583:353675-353992 [Vibrio cholerae O395]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36