Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2959397..2960047 Replicon chromosome
Accession NZ_CP092828
Organism Klebsiella michiganensis strain HOBD-7

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag MJ905_RS13990 Protein ID WP_014227314.1
Coordinates 2959706..2960047 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag MJ905_RS13985 Protein ID WP_014227313.1
Coordinates 2959397..2959696 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ905_RS13965 (MJ905_13960) 2955282..2956001 - 720 WP_076752428.1 phosphonate utilization transcriptional regulator PhnR -
MJ905_RS13970 (MJ905_13965) 2956142..2957245 + 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
MJ905_RS13975 (MJ905_13970) 2957255..2958064 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
MJ905_RS13980 (MJ905_13975) 2958097..2959278 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
MJ905_RS13985 (MJ905_13980) 2959397..2959696 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
MJ905_RS13990 (MJ905_13985) 2959706..2960047 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ905_RS13995 (MJ905_13990) 2960298..2960750 - 453 WP_014839842.1 DUF1198 domain-containing protein -
MJ905_RS14000 (MJ905_13995) 2961199..2962590 - 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
MJ905_RS14005 (MJ905_14000) 2962736..2964055 - 1320 WP_014227316.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T237234 WP_014227314.1 NZ_CP092828:c2960047-2959706 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T237234 NZ_LR822060:c174760-174665 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT237234 WP_014227313.1 NZ_CP092828:c2959696-2959397 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT237234 NZ_LR822060:174580-174637 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References