Detailed information of TA system TA237168

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132679..132932
• Replicon: plasmid pXH1508-2
• Accession: NZ_CP092788
• Organism: Klebsiella pneumoniae strain XH1508

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: ML259_RS29015
• Protein ID: WP_001312851.1
• Coordinates: 132783..132932 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	132679..132738 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ML259_RS28975 (128061)	128061..128405	-	345	Protein_162	IS6-like element IS26 family transposase	-
ML259_RS28980 (128457)	128457..129161	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ML259_RS28985 (129186)	129186..129386	+	201	WP_072354025.1	hypothetical protein	-
ML259_RS28990 (129406)	129406..130152	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
ML259_RS28995 (130207)	130207..130767	+	561	WP_000139328.1	fertility inhibition protein FinO	-
ML259_RS29000 (130899)	130899..131099	+	201	WP_015059022.1	hypothetical protein	-
ML259_RS29005 (131485)	131485..132084	+	600	WP_032083981.1	PIN domain-containing protein	-
ML259_RS29010 (132146)	132146..132478	+	333	WP_152916585.1	hypothetical protein	-
- (132679)	132679..132738	-	60	NuclAT_1	-	Antitoxin
- (132679)	132679..132738	-	60	NuclAT_1	-	Antitoxin
- (132679)	132679..132738	-	60	NuclAT_1	-	Antitoxin
- (132679)	132679..132738	-	60	NuclAT_1	-	Antitoxin
ML259_RS29015 (132783)	132783..132932	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
ML259_RS29020 (133216)	133216..133464	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133775	133775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T237168 WP_001312851.1 NZ_CP092788:132783-132932 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T237168 NZ_LR778300:c94775-94386 [Thermococcus sp. 2319x1]
GTGATAGTAATTGATGCATCTGCTCTCGCTAAAGTTATCCTTCAAGAAGAGAACTGGGAAAGTATCCCACTAACGGAAAA
AACTGCAACGCTGGATTACGCTCTCGTTGAAGCATTAAACGCTATTTGGAAGGCTGTTATCCAAAAACGGCTAGATGAAG
AAGACGCAAAAAACAGAACTGAAGCACTGAAGTATCTCGCCAAAAGCCTGTTGCTCTTCGAAGCCAAGAATTACTTTGAG
CGTGCTTTGGAGATAGCGCTGAAGGAAAAAATTACCGTTTACGATGCTCTCTACATAGCCCTTGCGGAAGAACTAAAAGC
TGAGTTTTATACCCCTGACGTTAAGCAGTTTGAGGCAGCGAAAAAATACGTGAGAGTAAAGCTCATTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT237168 NZ_CP092788:c132738-132679 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin