Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 353929..354575 Replicon chromosome
Accession NZ_CP092759
Organism Klebsiella pneumoniae strain bat16

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag MKZ45_RS01625 Protein ID WP_002920560.1
Coordinates 353929..354276 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MKZ45_RS01630 Protein ID WP_002920557.1
Coordinates 354276..354575 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ45_RS01615 (MKZ45_01615) 349855..351288 + 1434 WP_002920564.1 glycogen synthase GlgA -
MKZ45_RS01620 (MKZ45_01620) 351306..353753 + 2448 WP_002920561.1 glycogen phosphorylase -
MKZ45_RS01625 (MKZ45_01625) 353929..354276 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKZ45_RS01630 (MKZ45_01630) 354276..354575 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MKZ45_RS01635 (MKZ45_01635) 354638..356146 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MKZ45_RS01640 (MKZ45_01640) 356351..356680 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MKZ45_RS01645 (MKZ45_01645) 356731..357561 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MKZ45_RS01650 (MKZ45_01650) 357611..358369 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T237088 WP_002920560.1 NZ_CP092759:353929-354276 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T237088 NZ_LR778150:c119564-119139 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACGCATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAGTTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATGTTTTACGGGCGCGACAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT237088 WP_002920557.1 NZ_CP092759:354276-354575 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT237088 NZ_LR778150:c119930-119595 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGACTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References