Detailed information of TA system TA023708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 355936..356551
• Replicon: chromosome II
• Accession: NC_012580
• Organism: Vibrio cholerae M66-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: VCM66_RS15025
• Protein ID: WP_001162670.1
• Coordinates: 355936..356223 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VCM66_RS15030
• Protein ID: WP_001232701.1
• Coordinates: 356234..356551 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCM66_RS14995	351428..351685	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCM66_RS15000	351673..351975	+	303	WP_000229318.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCM66_RS18795	352264..352488	+	225	WP_001894423.1	DUF3709 domain-containing protein	-
VCM66_RS18800	352932..353051	+	120	WP_071919600.1	DUF645 family protein	-
VCM66_RS19335	353055..353108	+	54	Protein_329	DUF645 family protein	-
VCM66_RS15010	353710..354714	+	1005	WP_000964931.1	LD-carboxypeptidase	-
VCM66_RS15020	355489..355669	+	181	Protein_331	DUF645 family protein	-
VCM66_RS15025	355936..356223	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VCM66_RS15030	356234..356551	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
VCM66_RS19550	356548..356658	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
VCM66_RS15035	356776..357054	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
VCM66_RS19350	357306..357512	+	207	Protein_336	DUF3709 domain-containing protein	-
VCM66_RS18830	357712..357813	+	102	WP_001921603.1	hypothetical protein	-
VCM66_RS15045	357803..358189	+	387	WP_000703163.1	VOC family protein	-
VCM66_RS15050	358384..358635	+	252	WP_001890111.1	TIGR03643 family protein	-
VCM66_RS18835	358608..358757	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
VCM66_RS15055	358809..359162	+	354	WP_000384365.1	hypothetical protein	-
VCM66_RS15060	359374..359616	+	243	WP_000107461.1	hypothetical protein	-
VCM66_RS15065	359868..360146	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
VCM66_RS19555	360521..360664	+	144	Protein_344	DUF645 family protein	-
VCM66_RS19560	360718..360756	+	39	WP_082798268.1	hypothetical protein	-
VCM66_RS15075	360968..361435	+	468	WP_001289288.1	OsmC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	305869..409778	103909
	-	inside	Integron	-	-	310949..409402	98453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T23708 WP_001162670.1 NC_012580:355936-356223 [Vibrio cholerae M66-2]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T23708 NC_012580:355936-356223 [Vibrio cholerae M66-2]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT23708 WP_001232701.1 NC_012580:356234-356551 [Vibrio cholerae M66-2]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT23708 NC_012580:356234-356551 [Vibrio cholerae M66-2]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36