Detailed information of TA system TA237040

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22728..22981
• Replicon: plasmid unnamed1
• Accession: NZ_CP092718
• Organism: Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MKX18_RS27315
• Protein ID: WP_001312851.1
• Coordinates: 22832..22981 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	22728..22787 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKX18_RS27275 (18067)	18067..18453	+	387	Protein_27	relaxosome protein TraM	-
MKX18_RS27280 (18506)	18506..19210	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MKX18_RS27285 (19235)	19235..19435	+	201	WP_072354025.1	hypothetical protein	-
MKX18_RS27290 (19455)	19455..20201	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MKX18_RS27295 (20256)	20256..20816	+	561	WP_000139328.1	fertility inhibition protein FinO	-
MKX18_RS27300 (20948)	20948..21148	+	201	WP_015059022.1	hypothetical protein	-
MKX18_RS27305 (21534)	21534..22133	+	600	WP_032083981.1	PIN domain-containing protein	-
MKX18_RS27310 (22195)	22195..22527	+	333	WP_152916585.1	hypothetical protein	-
- (22728)	22728..22787	-	60	NuclAT_1	-	Antitoxin
- (22728)	22728..22787	-	60	NuclAT_1	-	Antitoxin
- (22728)	22728..22787	-	60	NuclAT_1	-	Antitoxin
- (22728)	22728..22787	-	60	NuclAT_1	-	Antitoxin
MKX18_RS27315 (22832)	22832..22981	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
MKX18_RS27320 (23265)	23265..23513	+	249	WP_000083837.1	replication regulatory protein RepA	-
MKX18_RS27325 (23628)	23628..23806	+	179	Protein_37	protein CopA/IncA	-
MKX18_RS27330 (23825)	23825..24682	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
MKX18_RS27335 (25621)	25621..26274	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MKX18_RS27340 (26367)	26367..26624	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MKX18_RS27345 (26557)	26557..26958	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MKX18_RS27350 (27207)	27207..27622	+	416	Protein_42	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133346	133346
	-	flank	IS/Tn	-	-	18506..19210	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T237040 WP_001312851.1 NZ_CP092718:22832-22981 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T237040 NZ_LR778147:2575119-2575373 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT237040 NZ_CP092718:c22787-22728 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin