Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3276981..3277780 Replicon chromosome
Accession NZ_CP092702
Organism Escherichia coli strain S-P-C-029.01

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MJ592_RS15990 Protein ID WP_000347273.1
Coordinates 3277316..3277780 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MJ592_RS15985 Protein ID WP_001307405.1
Coordinates 3276981..3277316 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ592_RS15970 (3272766) 3272766..3273536 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
MJ592_RS15975 (3273552) 3273552..3274886 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MJ592_RS15980 (3275261) 3275261..3276832 + 1572 WP_001273753.1 galactarate dehydratase -
MJ592_RS15985 (3276981) 3276981..3277316 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MJ592_RS15990 (3277316) 3277316..3277780 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MJ592_RS15995 (3277835) 3277835..3278644 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MJ592_RS16000 (3278893) 3278893..3280173 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MJ592_RS16005 (3280196) 3280196..3280669 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MJ592_RS16010 (3280680) 3280680..3281051 + 372 Protein_3144 PTS sugar transporter subunit IIC -
MJ592_RS16015 (3281047) 3281047..3281604 + 558 Protein_3145 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3267833..3288764 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T236997 WP_000347273.1 NZ_CP092702:3277316-3277780 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T236997 NZ_LR778145:c139890-139579 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236997 WP_001307405.1 NZ_CP092702:3276981-3277316 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236997 NZ_LR778145:c139578-139288 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References