Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3233707..3234434 Replicon chromosome
Accession NZ_CP092702
Organism Escherichia coli strain S-P-C-029.01

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MJ592_RS15765 Protein ID WP_000550189.1
Coordinates 3234120..3234434 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MJ592_RS15760 Protein ID WP_000560266.1
Coordinates 3233707..3234123 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ592_RS15750 (3228867) 3228867..3231218 + 2352 WP_000695487.1 alpha-glucosidase -
MJ592_RS15755 (3231644) 3231644..3233662 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MJ592_RS15760 (3233707) 3233707..3234123 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MJ592_RS15765 (3234120) 3234120..3234434 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MJ592_RS15770 (3234718) 3234718..3235854 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MJ592_RS15775 (3235939) 3235939..3236442 + 504 WP_001333820.1 M48 family metallopeptidase -
MJ592_RS15780 (3236519) 3236519..3237211 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
MJ592_RS15785 (3237290) 3237290..3238276 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T236996 WP_000550189.1 NZ_CP092702:c3234434-3234120 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T236996 NZ_LR778143:4270722-4270829 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT236996 WP_000560266.1 NZ_CP092702:c3234123-3233707 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT236996 NZ_LR778143:c4270672-4270609 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References