Detailed information of TA system TA023698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4942671..4943326
• Replicon: chromosome
• Accession: NC_012560
• Organism: Azotobacter vinelandii DJ

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M9YJW4
• Locus tag: AVIN_RS22335
• Protein ID: WP_012703346.1
• Coordinates: 4942982..4943326 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AVIN_RS22330
• Protein ID: WP_012703345.1
• Coordinates: 4942671..4942985 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVIN_RS25685	4938413..4939156	-	744	WP_012703339.1	HNH endonuclease	-
AVIN_RS22320	4939296..4940264	+	969	WP_012703340.1	IS110 family transposase	-
AVIN_RS22325	4940481..4941245	-	765	WP_012703341.1	class I SAM-dependent methyltransferase	-
AVIN_RS25690	4941539..4942402	-	864	WP_012703343.1	hypothetical protein	-
AVIN_RS22330	4942671..4942985	-	315	WP_012703345.1	helix-turn-helix domain-containing protein	Antitoxin
AVIN_RS22335	4942982..4943326	-	345	WP_012703346.1	hypothetical protein	Toxin
AVIN_RS22340	4943404..4944777	-	1374	WP_012703347.1	aspartate aminotransferase family protein	-
AVIN_RS22345	4944846..4946207	-	1362	WP_012703348.1	glutamine synthetase family protein	-
AVIN_RS22350	4946233..4946985	-	753	WP_012703349.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4939452..4940264	812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-88)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13080.00 Da
• Isoelectric Point: 9.8648

>T23698 WP_012703346.1 NC_012560:c4943326-4942982 [Azotobacter vinelandii DJ]
MKALFIETTSFTATVGDYLTDDEYRQLQGELLKNPDAGDVMPRTGGFRKLRWADGRRGKGKRGGLRVIYYWLLGDGQFWM
FAIYDKNELENLTAEQEKLLKQAITAELKKRGAK

Nucleotide

• Length: 345 bp

>T23698 NC_012560:c4943326-4942982 [Azotobacter vinelandii DJ]
ATGAAAGCCCTCTTCATCGAAACCACGAGCTTTACCGCCACGGTCGGCGACTACCTGACCGACGACGAATATCGGCAACT
CCAGGGGGAACTGCTGAAGAACCCCGATGCCGGCGACGTCATGCCACGCACGGGCGGCTTCCGCAAGCTGCGCTGGGCCG
ATGGGCGCAGAGGGAAGGGAAAGCGTGGTGGCTTGCGCGTCATCTACTACTGGCTGCTCGGCGATGGCCAGTTCTGGATG
TTCGCCATCTACGACAAAAATGAGCTGGAGAACCTCACTGCCGAGCAGGAGAAGCTGCTCAAGCAGGCCATCACCGCAGA
GCTGAAAAAGCGAGGTGCCAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11995.99 Da
• Isoelectric Point: 10.4600

>AT23698 WP_012703345.1 NC_012560:c4942985-4942671 [Azotobacter vinelandii DJ]
VKKRDLFAEMMQGIGEMEAHREGKITLRQYEVEERPAPEVSASEIVALRKKMHMSQQVFARRFRISTGTLRKWEQAKAKP
NAQAAVLIRLVEQYPDMAERLAAI

Nucleotide

• Length: 315 bp

>AT23698 NC_012560:c4942985-4942671 [Azotobacter vinelandii DJ]
GTGAAAAAGCGTGATTTGTTTGCCGAGATGATGCAGGGCATCGGCGAGATGGAGGCGCACCGTGAGGGCAAGATCACTCT
GCGCCAATACGAGGTCGAAGAAAGGCCGGCGCCGGAGGTGTCGGCGAGCGAGATCGTCGCCCTGCGCAAGAAAATGCACA
TGTCCCAACAGGTGTTTGCCCGCCGCTTCCGCATCAGCACGGGCACCCTGCGCAAATGGGAGCAGGCCAAGGCCAAGCCG
AACGCACAGGCTGCCGTTCTCATCAGACTGGTCGAGCAGTACCCCGACATGGCCGAAAGGCTGGCCGCGATCTGA

Structures

Toxin

• AlphaFold DB: M9YJW4