Detailed information of TA system TA236962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 31318..32029
• Replicon: plasmid pMFDS1011716
• Accession: NZ_CP092691
• Organism: Salmonella enterica subsp. enterica serovar Thompson strain MFDS1011716

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: MKV11_RS23670
• Protein ID: WP_000162415.1
• Coordinates: 31318..31620 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKV11_RS23675
• Protein ID: WP_000806445.1
• Coordinates: 31691..32029 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKV11_RS23640 (MKV11_23635)	26337..26987	-	651	WP_032153356.1	hypothetical protein	-
MKV11_RS23645 (MKV11_23640)	27001..27906	-	906	WP_001258014.1	recombination-associated protein RdgC	-
MKV11_RS23650 (MKV11_23645)	27966..28619	-	654	WP_271232211.1	baseplate protein	-
MKV11_RS23655 (MKV11_23650)	28616..29596	-	981	WP_271232212.1	tail length tape measure protein	-
MKV11_RS23660 (MKV11_23655)	29600..30367	-	768	WP_000203290.1	hypothetical protein	-
MKV11_RS23665 (MKV11_23660)	30364..31155	-	792	WP_000532305.1	hypothetical protein	-
MKV11_RS23670 (MKV11_23665)	31318..31620	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKV11_RS23675 (MKV11_23670)	31691..32029	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
MKV11_RS23680 (MKV11_23675)	32086..32271	+	186	Protein_35	hypothetical protein	-
MKV11_RS23685 (MKV11_23680)	32308..33036	+	729	WP_000986754.1	hypothetical protein	-
MKV11_RS23690 (MKV11_23685)	33070..34263	-	1194	WP_001112633.1	hypothetical protein	-
MKV11_RS23695 (MKV11_23690)	34256..34585	-	330	WP_000542383.1	hypothetical protein	-
MKV11_RS23700 (MKV11_23695)	34914..35567	-	654	WP_000410952.1	hypothetical protein	-
MKV11_RS23705 (MKV11_23700)	35913..36734	+	822	WP_001427910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92357	92357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T236962 WP_000162415.1 NZ_CP092691:31318-31620 [Salmonella enterica subsp. enterica serovar Thompson]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T236962 NZ_LR778142:c1994529-1994251 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT236962 WP_000806445.1 NZ_CP092691:31691-32029 [Salmonella enterica subsp. enterica serovar Thompson]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT236962 NZ_LR778142:c1994251-1993967 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3