Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 354811..355457 Replicon chromosome
Accession NZ_CP092648
Organism Klebsiella pneumoniae strain KP7728

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag MJ615_RS01640 Protein ID WP_016529833.1
Coordinates 354811..355158 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MJ615_RS01645 Protein ID WP_002920557.1
Coordinates 355158..355457 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ615_RS01630 (350737) 350737..352170 + 1434 WP_240289432.1 glycogen synthase GlgA -
MJ615_RS01635 (352188) 352188..354635 + 2448 WP_002920561.1 glycogen phosphorylase -
MJ615_RS01640 (354811) 354811..355158 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ615_RS01645 (355158) 355158..355457 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MJ615_RS01650 (355520) 355520..357028 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
MJ615_RS01655 (357233) 357233..357562 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MJ615_RS01660 (357613) 357613..358443 + 831 WP_024622927.1 rhomboid family intramembrane serine protease GlpG -
MJ615_RS01665 (358493) 358493..359251 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T236875 WP_016529833.1 NZ_CP092648:354811-355158 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T236875 NZ_LR740777:c109846-109541 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT236875 WP_002920557.1 NZ_CP092648:355158-355457 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT236875 NZ_LR740777:c110066-109848 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References