Detailed information of TA system TA236869

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4724402..4724773
• Replicon: chromosome
• Accession: NZ_CP092647
• Organism: Escherichia coli strain EC5502

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: MJ614_RS25060
• Protein ID: WP_001317028.1
• Coordinates: 4724579..4724773 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4724402..4724580 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ614_RS25030 (4720154)	4720154..4720327	+	174	WP_001296046.1	protein YnaL	-
MJ614_RS25035 (4720357)	4720357..4721730	+	1374	WP_000123738.1	ATP-dependent RNA helicase DbpA	-
MJ614_RS25040 (4721859)	4721859..4722794	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
MJ614_RS25045 (4722846)	4722846..4724081	-	1236	WP_000040858.1	site-specific integrase	-
MJ614_RS25050 (4724083)	4724083..4724298	-	216	WP_000079604.1	excisionase XisR	-
- (4724402)	4724402..4724580	+	179	NuclAT_0	-	Antitoxin
- (4724402)	4724402..4724580	+	179	NuclAT_0	-	Antitoxin
- (4724402)	4724402..4724580	+	179	NuclAT_0	-	Antitoxin
- (4724402)	4724402..4724580	+	179	NuclAT_0	-	Antitoxin
MJ614_RS25055 (4724377)	4724377..4724586	-	210	WP_021565074.1	double-strand break reduction protein RcbA	-
MJ614_RS25060 (4724579)	4724579..4724773	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MJ614_RS25065 (4724830)	4724830..4725639	-	810	WP_000166319.1	recombination protein RecT	-
MJ614_RS25070 (4725632)	4725632..4728232	-	2601	WP_047667559.1	exodeoxyribonuclease VIII	-
MJ614_RS25075 (4728334)	4728334..4728609	-	276	WP_000632297.1	protein RacC	-
MJ614_RS25080 (4728684)	4728684..4728854	-	171	WP_001352098.1	YdaE family protein	-
MJ614_RS25085 (4728854)	4728854..4729075	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4715587..4773068	57481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T236869 WP_001317028.1 NZ_CP092647:c4724773-4724579 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T236869 NZ_LR740776:c4658380-4658006 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT236869 NZ_CP092647:4724402-4724580 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGAAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin