Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3544164..3544705 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag MJ614_RS19230 Protein ID WP_000615982.1
Coordinates 3544164..3544442 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MJ614_RS19235 Protein ID WP_000729704.1
Coordinates 3544445..3544705 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS19215 (3541746) 3541746..3542324 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
MJ614_RS19220 (3542530) 3542530..3543297 + 768 WP_000333380.1 class II glutamine amidotransferase -
MJ614_RS19225 (3543268) 3543268..3544008 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MJ614_RS19230 (3544164) 3544164..3544442 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MJ614_RS19235 (3544445) 3544445..3544705 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MJ614_RS19240 (3544891) 3544891..3545664 + 774 WP_000543899.1 C40 family peptidase -
MJ614_RS19245 (3545721) 3545721..3546077 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
MJ614_RS19250 (3546070) 3546070..3546348 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
MJ614_RS19255 (3546453) 3546453..3548165 - 1713 Protein_3383 flagellar biosynthesis protein FlhA -
MJ614_RS19260 (3548137) 3548137..3548922 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T236866 WP_000615982.1 NZ_CP092647:c3544442-3544164 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T236866 NZ_LR740776:4049856-4050263 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCACGGTAAGCATCCGGAGAAACGATGAAACTTTCCCTGATGCTGGAAGCCGAC
AGAATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT236866 WP_000729704.1 NZ_CP092647:c3544705-3544445 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT236866 NZ_LR740776:4049398-4049766 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References