Detailed information of TA system TA236862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2653850..2654452
• Replicon: chromosome
• Accession: NZ_CP092647
• Organism: Escherichia coli strain EC5502

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MJ614_RS15115
• Protein ID: WP_000897305.1
• Coordinates: 2653850..2654161 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ614_RS15120
• Protein ID: WP_000356397.1
• Coordinates: 2654162..2654452 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ614_RS15085 (2648880)	2648880..2649665	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
MJ614_RS15090 (2649764)	2649764..2650363	+	600	WP_001295269.1	glucose-1-phosphatase	-
MJ614_RS15095 (2650357)	2650357..2651229	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MJ614_RS15100 (2651226)	2651226..2651663	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MJ614_RS15105 (2651708)	2651708..2652649	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MJ614_RS15110 (2652713)	2652713..2653621	-	909	WP_001162704.1	alpha/beta hydrolase	-
MJ614_RS15115 (2653850)	2653850..2654161	+	312	WP_000897305.1	hypothetical protein	Toxin
MJ614_RS15120 (2654162)	2654162..2654452	+	291	WP_000356397.1	NadS family protein	Antitoxin
MJ614_RS15125 (2655038)	2655038..2655256	+	219	WP_001611167.1	CopG family transcriptional regulator	-
MJ614_RS15130 (2655475)	2655475..2655717	+	243	WP_001086388.1	protein YiiF	-
MJ614_RS15135 (2656047)	2656047..2656976	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MJ614_RS15140 (2656973)	2656973..2657608	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MJ614_RS15145 (2657605)	2657605..2658507	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T236862 WP_000897305.1 NZ_CP092647:2653850-2654161 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T236862 NZ_LR740776:c2159615-2159361 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT236862 WP_000356397.1 NZ_CP092647:2654162-2654452 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT236862 NZ_LR740776:c2159863-2159612 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7