Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1859037..1859836 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag MJ614_RS11255 Protein ID WP_000347266.1
Coordinates 1859372..1859836 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MJ614_RS11250 Protein ID WP_001307405.1
Coordinates 1859037..1859372 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS11235 (1854822) 1854822..1855592 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
MJ614_RS11240 (1855608) 1855608..1856942 - 1335 WP_000599640.1 galactarate/glucarate/glycerate transporter GarP -
MJ614_RS11245 (1857317) 1857317..1858888 + 1572 WP_001273741.1 galactarate dehydratase -
MJ614_RS11250 (1859037) 1859037..1859372 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MJ614_RS11255 (1859372) 1859372..1859836 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MJ614_RS11260 (1859891) 1859891..1860700 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MJ614_RS11265 (1860949) 1860949..1862229 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
MJ614_RS11270 (1862252) 1862252..1862725 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MJ614_RS11275 (1862736) 1862736..1863515 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MJ614_RS11280 (1863505) 1863505..1864383 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
MJ614_RS11285 (1864401) 1864401..1864835 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1848381..1859836 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T236860 WP_000347266.1 NZ_CP092647:1859372-1859836 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T236860 NZ_LR740776:c1875357-1875255 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236860 WP_001307405.1 NZ_CP092647:1859037-1859372 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236860 NZ_LR740776:1875217-1875485 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References