Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3573067..3573608 Replicon chromosome
Accession NZ_CP092639
Organism Escherichia coli strain HS

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag MJ392_RS17305 Protein ID WP_000615976.1
Coordinates 3573330..3573608 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MJ392_RS17300 Protein ID WP_000729704.1
Coordinates 3573067..3573327 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ392_RS17275 (3568850) 3568850..3569635 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
MJ392_RS17280 (3569607) 3569607..3571319 + 1713 Protein_3396 flagellar biosynthesis protein FlhA -
MJ392_RS17285 (3571424) 3571424..3571702 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
MJ392_RS17290 (3571695) 3571695..3572051 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
MJ392_RS17295 (3572108) 3572108..3572881 - 774 WP_000543897.1 C40 family peptidase -
MJ392_RS17300 (3573067) 3573067..3573327 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MJ392_RS17305 (3573330) 3573330..3573608 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MJ392_RS17310 (3573764) 3573764..3574504 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MJ392_RS17315 (3574475) 3574475..3575242 - 768 WP_000333380.1 class II glutamine amidotransferase -
MJ392_RS17320 (3575448) 3575448..3576026 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T236841 WP_000615976.1 NZ_CP092639:3573330-3573608 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T236841 NZ_LR740758:c5051925-5051584 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCACTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT236841 WP_000729704.1 NZ_CP092639:3573067-3573327 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT236841 NZ_LR740758:5051920-5051996 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References