Detailed information of TA system TA023683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3320110..3320729
• Replicon: chromosome
• Accession: NC_012522
• Organism: Rhodococcus opacus B4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: C1B6N0
• Locus tag: ROP_RS15260
• Protein ID: WP_012690289.1
• Coordinates: 3320448..3320729 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ROP_RS15255
• Protein ID: WP_012690288.1
• Coordinates: 3320110..3320403 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROP_RS15240	3316036..3316824	+	789	WP_012690282.1	alpha/beta hydrolase	-
ROP_RS15245	3317856..3318803	-	948	WP_012690285.1	hypothetical protein	-
ROP_RS15250	3319110..3320008	+	899	Protein_3045	recombinase family protein	-
ROP_RS15255	3320110..3320403	-	294	WP_012690288.1	HigA family addiction module antidote protein	Antitoxin
ROP_RS15260	3320448..3320729	-	282	WP_012690289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ROP_RS15265	3320892..3321146	-	255	Protein_3048	hypothetical protein	-
ROP_RS43715	3321218..3321379	+	162	WP_162471088.1	hypothetical protein	-
ROP_RS41185	3321367..3321900	-	534	Protein_3050	alkene reductase	-
ROP_RS15270	3321960..3322955	-	996	WP_012690292.1	LLM class flavin-dependent oxidoreductase	-
ROP_RS15275	3323035..3324072	-	1038	WP_043824802.1	MFS transporter	-
ROP_RS15280	3324150..3324512	+	363	WP_005250705.1	MerR family transcriptional regulator	-
ROP_RS15285	3324674..3325669	+	996	WP_043824804.1	nuclear transport factor 2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 9.5432

>T23683 WP_012690289.1 NC_012522:c3320729-3320448 [Rhodococcus opacus B4]
VIESFADKETERVFRRQFSKKVPQDIQRIARRRLLLIEAATDVNDLRVPPGNRLEKLSGDRVGQYSVRINDQYRICFIWR
ATAAYDVEITDYH

Nucleotide

• Length: 282 bp

>T23683 NC_012522:c3320729-3320448 [Rhodococcus opacus B4]
GTGATCGAATCGTTCGCCGACAAGGAGACCGAGCGAGTCTTTCGCCGGCAGTTCTCGAAGAAGGTCCCGCAGGACATTCA
GCGGATCGCGCGCCGGCGTCTCCTGTTGATCGAGGCGGCCACCGACGTCAACGACCTGCGGGTGCCCCCAGGCAACCGCC
TGGAGAAGCTCTCCGGGGACCGGGTGGGCCAGTACAGCGTCCGGATCAACGATCAGTACCGCATCTGTTTCATCTGGCGC
GCCACCGCCGCATACGACGTGGAGATCACCGACTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10775.37 Da
• Isoelectric Point: 5.8064

>AT23683 WP_012690288.1 NC_012522:c3320403-3320110 [Rhodococcus opacus B4]
MTTDLSPIHPGEILREDFLEPFGITQNRLATIIGVPPRRINEIVLGKRSITADTALRLAQAFGNSAQFWLNIQDRYELDL
AADKLGDTLAKITRLSA

Nucleotide

• Length: 294 bp

>AT23683 NC_012522:c3320403-3320110 [Rhodococcus opacus B4]
ATGACTACAGACCTGAGCCCCATTCACCCCGGCGAGATCCTCCGCGAGGACTTCCTCGAGCCTTTCGGTATCACGCAGAA
CCGCCTGGCCACGATCATCGGTGTGCCGCCCCGGCGGATCAACGAGATCGTGCTCGGCAAGCGGTCGATCACCGCAGACA
CCGCGCTGCGGCTGGCCCAGGCGTTCGGCAACAGCGCCCAGTTCTGGCTCAACATCCAGGACCGCTACGAGCTCGACCTT
GCGGCCGACAAGCTCGGCGACACCCTCGCCAAGATCACGCGCCTCTCGGCATAG

Structures

Toxin

• AlphaFold DB: C1B6N0