Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 633102..633829 Replicon chromosome
Accession NZ_CP092639
Organism Escherichia coli strain HS

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MJ392_RS03145 Protein ID WP_000550189.1
Coordinates 633102..633416 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MJ392_RS03150 Protein ID WP_000560255.1
Coordinates 633413..633829 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ392_RS03125 (629268) 629268..630254 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
MJ392_RS03130 (630333) 630333..631016 - 684 WP_001183041.1 vancomycin high temperature exclusion protein -
MJ392_RS03135 (631093) 631093..631596 - 504 WP_001295542.1 M48 family metallopeptidase -
MJ392_RS03140 (631681) 631681..632817 + 1137 WP_000018663.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MJ392_RS03145 (633102) 633102..633416 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MJ392_RS03150 (633413) 633413..633829 + 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MJ392_RS03155 (633874) 633874..635892 - 2019 WP_000121450.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MJ392_RS03160 (636218) 636218..638569 - 2352 WP_000695494.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T236822 WP_000550189.1 NZ_CP092639:633102-633416 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T236822 NZ_LR740758:1361595-1361697 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT236822 WP_000560255.1 NZ_CP092639:633413-633829 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT236822 NZ_LR740758:c1361735-1361467 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References