Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 584480..585279 Replicon chromosome
Accession NZ_CP092639
Organism Escherichia coli strain HS

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MJ392_RS02900 Protein ID WP_000347251.1
Coordinates 584480..584944 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MJ392_RS02905 Protein ID WP_001307405.1
Coordinates 584944..585279 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ392_RS02870 (579481) 579481..579915 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MJ392_RS02875 (579933) 579933..580811 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MJ392_RS02880 (580801) 580801..581580 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MJ392_RS02885 (581591) 581591..582064 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MJ392_RS02890 (582087) 582087..583367 - 1281 WP_000681905.1 tagatose-bisphosphate aldolase subunit KbaZ -
MJ392_RS02895 (583616) 583616..584425 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MJ392_RS02900 (584480) 584480..584944 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MJ392_RS02905 (584944) 584944..585279 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MJ392_RS02910 (585428) 585428..586999 - 1572 WP_001273782.1 galactarate dehydratase -
MJ392_RS02915 (587374) 587374..588708 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MJ392_RS02920 (588724) 588724..589494 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T236821 WP_000347251.1 NZ_CP092639:c584944-584480 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T236821 NZ_LR740758:505096-505398 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236821 WP_001307405.1 NZ_CP092639:c585279-584944 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236821 NZ_LR740758:505434-505775 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References