Detailed information of TA system TA236820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5256325..5256927
• Replicon: chromosome
• Accession: NZ_CP092635
• Organism: Enterobacter cloacae strain EFN743

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Z1J7K2
• Locus tag: MKZ88_RS25350
• Protein ID: WP_058651837.1
• Coordinates: 5256616..5256927 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKZ88_RS25345
• Protein ID: WP_013099359.1
• Coordinates: 5256325..5256615 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKZ88_RS25330 (MKZ88_25330)	5253822..5254724	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
MKZ88_RS25335 (MKZ88_25335)	5254721..5255356	+	636	WP_023622447.1	formate dehydrogenase cytochrome b556 subunit	-
MKZ88_RS25340 (MKZ88_25340)	5255353..5256282	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
MKZ88_RS25345 (MKZ88_25345)	5256325..5256615	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
MKZ88_RS25350 (MKZ88_25350)	5256616..5256927	-	312	WP_058651837.1	hypothetical protein	Toxin
MKZ88_RS25355 (MKZ88_25355)	5257157..5258074	+	918	WP_058651835.1	alpha/beta hydrolase	-
MKZ88_RS25360 (MKZ88_25360)	5258087..5259028	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
MKZ88_RS25365 (MKZ88_25365)	5259073..5259510	-	438	WP_058651833.1	D-aminoacyl-tRNA deacylase	-
MKZ88_RS25370 (MKZ88_25370)	5259507..5260388	-	882	WP_013099364.1	virulence factor BrkB family protein	-
MKZ88_RS25375 (MKZ88_25375)	5260382..5260981	-	600	WP_013099365.1	glucose-1-phosphatase	-
MKZ88_RS25380 (MKZ88_25380)	5261101..5261901	-	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12191.14 Da
• Isoelectric Point: 9.4001

>T236820 WP_058651837.1 NZ_CP092635:c5256927-5256616 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHHTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T236820 NZ_LR740758:c475973-475755 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT236820 WP_013099359.1 NZ_CP092635:c5256615-5256325 [Enterobacter cloacae]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT236820 NZ_LR740758:c476373-475999 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7Z1J7K2