Detailed information of TA system TA023682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2994967..2995469
• Replicon: chromosome
• Accession: NC_012522
• Organism: Rhodococcus opacus B4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: C1B5A1
• Locus tag: ROP_RS13795
• Protein ID: WP_012689983.1
• Coordinates: 2994967..2995221 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C1B5A2
• Locus tag: ROP_RS13800
• Protein ID: WP_012689984.1
• Coordinates: 2995218..2995469 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROP_RS13780	2992292..2992918	-	627	WP_012689980.1	TetR/AcrR family transcriptional regulator	-
ROP_RS13785	2993028..2994236	+	1209	WP_043824729.1	DUF2236 domain-containing protein	-
ROP_RS13790	2994244..2994750	-	507	WP_012689982.1	DUF664 domain-containing protein	-
ROP_RS13795	2994967..2995221	-	255	WP_012689983.1	Txe/YoeB family addiction module toxin	Toxin
ROP_RS13800	2995218..2995469	-	252	WP_012689984.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
ROP_RS13810	2995815..2997956	-	2142	WP_012689986.1	MMPL family transporter	-
ROP_RS13815	2998056..2998490	+	435	WP_012689987.1	MarR family transcriptional regulator	-
ROP_RS13820	2998491..2999294	-	804	WP_012689988.1	hypothetical protein	-
ROP_RS13825	2999370..2999909	-	540	WP_012689989.1	lipoprotein LpqH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10218.61 Da
• Isoelectric Point: 8.8640

>T23682 WP_012689983.1 NC_012522:c2995221-2994967 [Rhodococcus opacus B4]
MKLVWDESAWEDYVWWQAQDRKIVKRINTLVKDIERNGNDGIGKPEPLKHGFQGYWSRRITDEHRLVYKIVDDQIRIAAC
RYHY

Nucleotide

• Length: 255 bp

>T23682 NC_012522:c2995221-2994967 [Rhodococcus opacus B4]
ATGAAACTTGTCTGGGACGAGTCCGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGCAAAATCGTCAAGCGGAT
CAACACGCTGGTCAAGGACATCGAACGCAACGGAAACGACGGAATCGGCAAACCTGAGCCCCTCAAACACGGCTTCCAGG
GCTACTGGTCCCGACGCATCACTGACGAGCACCGGCTGGTGTACAAGATCGTCGACGACCAGATCCGCATTGCGGCCTGT
CGTTACCACTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.50 Da
• Isoelectric Point: 4.5216

>AT23682 WP_012689984.1 NC_012522:c2995469-2995218 [Rhodococcus opacus B4]
MRTMTYSESRARYAEVLNAVVDDREEIVITRAGHEPVVMVSLDDYESLKEAAYLLRNPENARRLLASIDRLEAGGGTVRD
LAE

Nucleotide

• Length: 252 bp

>AT23682 NC_012522:c2995469-2995218 [Rhodococcus opacus B4]
ATGCGAACCATGACCTATTCGGAATCGCGCGCGAGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGATCGCGAAGAAAT
CGTGATCACGCGAGCGGGGCACGAACCGGTGGTGATGGTCTCCCTCGATGACTACGAGTCCCTGAAGGAAGCGGCCTATC
TGCTCAGGAATCCGGAGAACGCACGTCGGCTGTTGGCATCGATCGATCGTCTGGAAGCCGGCGGGGGAACTGTCCGGGAT
CTAGCTGAATGA

Structures

Toxin

• AlphaFold DB: C1B5A1

Antitoxin

• AlphaFold DB: C1B5A2