Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 30746..31476 Replicon chromosome
Accession NZ_CP092635
Organism Enterobacter cloacae strain EFN743

Toxin (Protein)


Gene name higB Uniprot ID A0A7Z1J9A0
Locus tag MKZ88_RS00155 Protein ID WP_020684666.1
Coordinates 30746..31060 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7Z1F3E4
Locus tag MKZ88_RS00160 Protein ID WP_058650614.1
Coordinates 31060..31476 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ88_RS00130 (MKZ88_00130) 26410..27498 + 1089 WP_058650558.1 cellulase family glycosylhydrolase -
MKZ88_RS00135 (MKZ88_00135) 27401..28585 - 1185 WP_023620624.1 multidrug efflux MFS transporter EmrD -
MKZ88_RS00140 (MKZ88_00140) 28761..29594 - 834 WP_029881854.1 EamA family transporter -
MKZ88_RS00145 (MKZ88_00145) 29657..30103 - 447 WP_013094803.1 GNAT family N-acetyltransferase -
MKZ88_RS00150 (MKZ88_00150) 30168..30257 - 90 WP_020684667.1 type I toxin-antitoxin system toxin TisB -
MKZ88_RS00155 (MKZ88_00155) 30746..31060 + 315 WP_020684666.1 type II toxin-antitoxin system HigB family toxin Toxin
MKZ88_RS00160 (MKZ88_00160) 31060..31476 + 417 WP_058650614.1 helix-turn-helix domain-containing protein Antitoxin
MKZ88_RS00165 (MKZ88_00165) 31623..31721 + 99 WP_071830013.1 ilvB operon leader peptide IvbL -
MKZ88_RS00170 (MKZ88_00170) 31826..33514 + 1689 WP_048972063.1 acetolactate synthase large subunit -
MKZ88_RS00175 (MKZ88_00175) 33518..33805 + 288 WP_001171659.1 acetolactate synthase small subunit -
MKZ88_RS00180 (MKZ88_00180) 33888..34481 + 594 WP_058650559.1 transcriptional regulator UhpA -
MKZ88_RS00185 (MKZ88_00185) 34478..35983 + 1506 WP_058650560.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(3-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12367.51 Da        Isoelectric Point: 10.2509

>T236802 WP_020684666.1 NZ_CP092635:30746-31060 [Enterobacter cloacae]
MHLISMKAILDAVIQFPQHREELLVLGRTLEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T236802 NZ_LR739069:c144182-143901 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15437.00 Da        Isoelectric Point: 5.7670

>AT236802 WP_058650614.1 NZ_CP092635:31060-31476 [Enterobacter cloacae]
MIVADAMKATHALVEAVPLLGEQPSEKDYNDALELVEYLLINEPNSPLLDIVCARISRYEANRPKIVALRKEMELVPVGI
AVLRTLMDQYKLTTSDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLASRFGVSPALFIE

Download         Length: 417 bp

>AT236802 NZ_LR739069:c144406-144179 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z1J9A0

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F3E4

References