236801

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5324248..5324843 Replicon chromosome
Accession NZ_CP092634
Organism Pseudomonas aeruginosa strain LS.2c

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag MKK05_RS24755 Protein ID WP_003113526.1
Coordinates 5324565..5324843 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag MKK05_RS24750 Protein ID WP_003111575.1
Coordinates 5324248..5324553 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKK05_RS24715 (MKK05_24715) 5319400..5320248 + 849 WP_009878189.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
MKK05_RS24725 (MKK05_24725) 5320415..5321356 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
MKK05_RS24730 (MKK05_24730) 5321473..5322087 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
MKK05_RS24735 (MKK05_24735) 5322129..5322713 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
MKK05_RS24740 (MKK05_24740) 5322754..5323854 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
MKK05_RS24750 (MKK05_24750) 5324248..5324553 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
MKK05_RS24755 (MKK05_24755) 5324565..5324843 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKK05_RS24760 (MKK05_24760) 5324896..5325024 - 129 Protein_4896 integrase -
MKK05_RS24765 (MKK05_24765) 5325172..5327400 + 2229 WP_021264080.1 TonB-dependent receptor -
MKK05_RS24770 (MKK05_24770) 5327470..5328117 - 648 WP_003095021.1 carbonate dehydratase -
MKK05_RS24775 (MKK05_24775) 5328179..5329417 - 1239 WP_003111578.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T236801 WP_003113526.1 NZ_CP092634:c5324843-5324565 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T236801 NZ_LR739068:c5595528-5595250 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT236801 WP_003111575.1 NZ_CP092634:c5324553-5324248 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT236801 NZ_LR739068:c5595238-5594933 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References