Detailed information of TA system TA236800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4686035..4686716
• Replicon: chromosome
• Accession: NZ_CP092634
• Organism: Pseudomonas aeruginosa strain LS.2c

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: MKK05_RS21805
• Protein ID: WP_003111825.1
• Coordinates: 4686351..4686716 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKK05_RS21800
• Protein ID: WP_003111824.1
• Coordinates: 4686035..4686358 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKK05_RS21775 (MKK05_21775)	4681942..4682580	+	639	WP_003109444.1	hypothetical protein	-
MKK05_RS21780 (MKK05_21780)	4682823..4683158	+	336	WP_003140884.1	TM2 domain-containing protein	-
MKK05_RS21785 (MKK05_21785)	4683332..4683850	+	519	WP_003140886.1	PAAR domain-containing protein	-
MKK05_RS21790 (MKK05_21790)	4683847..4684548	+	702	WP_031756445.1	VRR-NUC domain-containing protein	-
MKK05_RS21795 (MKK05_21795)	4684565..4685653	+	1089	WP_033995173.1	DUF3396 domain-containing protein	-
MKK05_RS21800 (MKK05_21800)	4686035..4686358	-	324	WP_003111824.1	XRE family transcriptional regulator	Antitoxin
MKK05_RS21805 (MKK05_21805)	4686351..4686716	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKK05_RS21810 (MKK05_21810)	4687009..4687269	-	261	WP_052155056.1	hypothetical protein	-
MKK05_RS21815 (MKK05_21815)	4687476..4687748	+	273	WP_024947448.1	hypothetical protein	-
MKK05_RS21820 (MKK05_21820)	4687779..4688204	-	426	WP_003140891.1	VOC family protein	-
MKK05_RS21825 (MKK05_21825)	4688305..4689189	+	885	WP_003140892.1	LysR substrate-binding domain-containing protein	-
MKK05_RS21830 (MKK05_21830)	4689162..4690115	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
MKK05_RS21835 (MKK05_21835)	4690336..4690770	+	435	WP_003114204.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T236800 WP_003111825.1 NZ_CP092634:c4686716-4686351 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T236800 NZ_LR739068:c5356085-5355675 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11887.72 Da
• Isoelectric Point: 5.8900

>AT236800 WP_003111824.1 NZ_CP092634:c4686358-4686035 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT236800 NZ_LR739068:c5356315-5356085 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Toxin

• AlphaFold DB: V6AKP0