Detailed information of TA system TA236794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 97622..98346
• Replicon: plasmid pML9ba2
• Accession: NZ_CP092633
• Organism: Klebsiella variicola subsp. variicola strain ML.9ba2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: MKK01_RS26960
• Protein ID: WP_023292165.1
• Coordinates: 98035..98346 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKK01_RS26955
• Protein ID: WP_049010942.1
• Coordinates: 97622..98038 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKK01_RS26925 (MKK01_26925)	93205..93612	+	408	WP_223170089.1	transglycosylase SLT domain-containing protein	-
MKK01_RS26930 (MKK01_26930)	93650..94180	-	531	WP_023292160.1	antirestriction protein	-
MKK01_RS26935 (MKK01_26935)	94805..95638	-	834	WP_240289753.1	N-6 DNA methylase	-
MKK01_RS26940 (MKK01_26940)	95684..95914	-	231	WP_240289754.1	DUF4496 domain-containing protein	-
MKK01_RS26945 (MKK01_26945)	96001..96348	-	348	WP_114501446.1	hypothetical protein	-
MKK01_RS26950 (MKK01_26950)	96414..96794	-	381	WP_240289755.1	hypothetical protein	-
MKK01_RS26955 (MKK01_26955)	97622..98038	-	417	WP_049010942.1	helix-turn-helix domain-containing protein	Antitoxin
MKK01_RS26960 (MKK01_26960)	98035..98346	-	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
MKK01_RS26965 (MKK01_26965)	98453..98677	-	225	WP_240289756.1	hypothetical protein	-
MKK01_RS26970 (MKK01_26970)	98688..98900	-	213	WP_049157092.1	hypothetical protein	-
MKK01_RS26975 (MKK01_26975)	98961..99317	-	357	WP_211754325.1	hypothetical protein	-
MKK01_RS26980 (MKK01_26980)	100147..100680	-	534	Protein_101	IS110 family transposase	-
MKK01_RS26985 (MKK01_26985)	100891..101406	+	516	WP_049156577.1	RNA polymerase sigma factor	-
MKK01_RS26990 (MKK01_26990)	101396..102376	+	981	WP_117110158.1	FecR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..166327	166327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T236794 WP_023292165.1 NZ_CP092633:c98346-98035 [Klebsiella variicola subsp. variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T236794 NZ_LR738724:c2002351-2002094 [Streptococcus suis]
ATGGGAATCCATTTTACAGAGGATGCTTGGGAAGACTATCTTTATTGGCAAGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAAATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCTTGGTCAAGGAGGATTGATGCGGAGAATCGGTTGATTTATATGGTGGAGGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGACCATTATGGGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15438.52 Da
• Isoelectric Point: 4.4711

>AT236794 WP_049010942.1 NZ_CP092633:c98038-97622 [Klebsiella variicola subsp. variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT236794 NZ_LR738724:c2002613-2002353 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGATGAGTTTGAGCCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGCGAGTGGGATAGTTTACAAGAAACCCTCA
CTGTTGCTCGGAACACCTATCTGTCTCAAAAAGTTTTGCGTGGTATGGCTCAAGTCAAAGCAGGGCAAACCCAAGAACGA
AACCTGATAGAGGCCGACTAG

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3