Detailed information of TA system TA236756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2512818..2513413
• Replicon: chromosome
• Accession: NZ_CP092629
• Organism: Pseudomonas aeruginosa strain HU20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MKF35_RS11610
• Protein ID: WP_003117425.1
• Coordinates: 2512818..2513096 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKF35_RS11615
• Protein ID: WP_003113527.1
• Coordinates: 2513108..2513413 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKF35_RS11590	2508244..2509482	+	1239	WP_003113524.1	dipeptidase	-
MKF35_RS11595	2509544..2510191	+	648	WP_003095021.1	carbonate dehydratase	-
MKF35_RS11600	2510261..2512489	-	2229	WP_003113525.1	TonB-dependent receptor	-
MKF35_RS11605	2512637..2512765	+	129	Protein_2290	integrase	-
MKF35_RS11610	2512818..2513096	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKF35_RS11615	2513108..2513413	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MKF35_RS11625	2513819..2514919	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MKF35_RS11630	2514960..2515544	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MKF35_RS11635	2515586..2516200	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
MKF35_RS11640	2516317..2517258	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MKF35_RS11650	2517425..2518273	-	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T236756 WP_003117425.1 NZ_CP092629:2512818-2513096 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T236756 NZ_LR735440:c81587-81378 [Staphylococcus epidermidis]
ATGTTAAACAGTAATATTGTATTTATAGATAACATTGAAACAAAGGAACAAGCACAAATGTTAAATGAACGTTTGTTAAA
TATGATTGGAGTACATACGGTATCAATCGATATAGAAAGAAATATGGTTAATGTTGAATATGAAACACCTACTAATTTAA
ATAGTATTGAAAAAGAAATTTACGACGCTGGATTTAAAGTACTTTCATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT236756 WP_003113527.1 NZ_CP092629:2513108-2513413 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT236756 NZ_LR735440:c81896-81603 [Staphylococcus epidermidis]
TTGGAAAATCAAAATCATGCCCATCATTCTGAGCAAGTTAAATTAAATTTAAAATCTCGATTAAATCGAATAGAAGGACA
AGTTAAAGCAATTAATCGAATGATTGATGAAGACGTCTACTGTGATGATGTATTAACTCAGATAAGAGCAACACGTTCAG
CACTTAATAGTGTTGCTACTAAGTTACTAGAACATCATATGAAAAGTTGTATTATGGAAAAAATAAATCAAGGTACTGAA
GAAGAAGCAATGGAAGAACTACTAGTGACATTTCAAAAACTAGTGAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5